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Enhancement in t1 lipase purification recovery using the novel construct pgex4t1/his-t1

The Geobacillus zalihae strain T1 produces a thermostable T1 lipase that could be used for industrial purposes. Previously, the GST-T1 lipase was purified through two chromatographic steps: affinity and ion exchange (IEX) but the recovery yield was only 33%. To improve the recovery yield to over 80%...

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Bibliographic Details
Main Authors:	Che Hussian, Che Haznie Ayu, Raja Abd Rahman, Raja Noor Zaliha, Leow, Adam Thean Chor, Salleh, Abu Bakar, Mohamad Ali, Mohd Shukuri, Latip, Wahhida
Format:	Article
Published:	Informa Uk Limited 2023
Online Access:	http://psasir.upm.edu.my/id/eprint/107690/ https://www.tandfonline.com/doi/full/10.1080/10826068.2023.2252052
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