Enhancement of a protocol purifying T1 lipase through molecular approach

• Main Authors: Che Hussian, Che Haznie Ayu, Raja Abdul Rahman, Raja Noor Zaliha, Leow, Adam Thean Chor, Salleh, Abu Bakar, Mohamad Ali, Mohd Shukuri
• Format: Article
• Language: English
• Published: PeerJ 2018
• Online Access: http://psasir.upm.edu.my/id/eprint/72578/1/Enhancement%20of%20a%20protocol%20purifying%20T1%20lipase%20through%20molecular%20approach.pdf; http://psasir.upm.edu.my/id/eprint/72578/; https://pubmed.ncbi.nlm.nih.gov/30479887/

T1 Lipase is a thermostable secretary protein of Geobacillus zalihae strain previously expressed in a prokaryotic system and purified using three-step purification: affinity 1, affinity 2, and ion exchange chromatography (IEX). This approach is time consuming and offers low purity and recovery yield...