Purified T1 lipase, a novel construct of T1 lipase and methods thereoF
The present invention relates to method to substitute second step affinity chromatography with ion exchange chromatography by site-directed mutagenesis at downstream region of GST sequences (H215R and G213R). This is to enable to diferentiate the isoelectric point (pI) value of GST from the pI value...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Patent |
| Published: |
2011
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/33123/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|