Reductive alkylation causes the formation of a molten globule-like intermediate structure in Geobacillus zalihae strain T1 thermostable lipase

• Main Authors: Cheong, Kok Whye, Leow, Adam Thean Chor, Raja Abdul Rahman, Raja Noor Zaliha, Basri, Mahiran, Abdul Rahman, Mohd Basyaruddin, Salleh, Abu Bakar
• Format: Article
• Language: English
• Published: Springer 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/22284/1/Reductive%20alkylation%20causes%20the%20formation%20of%20a%20molten%20globule.pdf; http://psasir.upm.edu.my/id/eprint/22284/; http://link.springer.com/article/10.1007/s12010-010-9140-8?view=classic

A thermostable lipase from Geobacillus zalihae strain T1 was chemically modified using propionaldehyde via reductive alkylation. The targeted alkylation sites were lysines, in which T1 lipase possessed 11 residues. Far-UV circular dichroism (CD) spectra of both native and alkylated enzyme showed a s...