Enhancement in t1 lipase purification recovery using the novel construct pgex4t1/his-t1

• Main Authors: Che Hussian, Che Haznie Ayu, Raja Abd Rahman, Raja Noor Zaliha, Leow, Adam Thean Chor, Salleh, Abu Bakar, Mohamad Ali, Mohd Shukuri, Latip, Wahhida
• 格式: Article
• 出版: Informa Uk Limited 2023
• 在线阅读: http://psasir.upm.edu.my/id/eprint/107690/; https://www.tandfonline.com/doi/full/10.1080/10826068.2023.2252052

The Geobacillus zalihae strain T1 produces a thermostable T1 lipase that could be used for industrial purposes. Previously, the GST-T1 lipase was purified through two chromatographic steps: affinity and ion exchange (IEX) but the recovery yield was only 33%. To improve the recovery yield to over 80%...