Improvement of thermal stability via outer-loop ion pair interaction of mutated T1 lipase from Geobacillus zalihae strain T1

• Main Authors: Ruslan, Rudzanna, Raja Abdul Rahman, Raja Noor Zaliha, Leow, Thean Chor, Mohamad Ali, Mohd Shukuri, Basri, Mahiran, Salleh, Abu Bakar
• Format: Article
• Language: English
• Published: MDPI AG 2012
• Online Access: http://psasir.upm.edu.my/id/eprint/27295/1/27295.pdf; http://psasir.upm.edu.my/id/eprint/27295/; http://www.mdpi.com/1422-0067/13/1/943

Mutant D311E and K344R were constructed using site-directed mutagenesis to introduce an additional ion pair at the inter-loop and the intra-loop, respectively, to determine the effect of ion pairs on the stability of T1 lipase isolated from Geobacillus zalihae. A series of purification steps was app...