Effects of lid 1 mutagenesis on lid displacement, catalytic performances and thermostability of cold-active Pseudomonas AMS8 lipase in toluene

• Main Authors: Yaacob, Norhayati, Ahmad Kamarudin, Nor Hafizah, Leow, Adam Thean Chor, Salleh, Abu Bakar, Raja Abdul Rahman, Raja Noor Zaliha, Mohamad Ali, Mohd Shukuri
• Format: Article
• Language: English
• Published: Elsevier 2019
• Online Access: http://psasir.upm.edu.my/id/eprint/79602/1/Effects%20of%20lid%201%20mutagenesis%20on%20lid%20displacement.pdf; http://psasir.upm.edu.my/id/eprint/79602/; https://www.sciencedirect.com/science/article/pii/S2001037018302435

Pseudomonas fluorescens AMS8 lipase lid 1 structure is rigid and holds unclear roles due to the absence of solvent-interactions. Lid 1 region was stabilized by 17 hydrogen bond linkages and displayed lower mean hydrophobicity (0.596) compared to MIS38 lipase. Mutating lid 1 residues, Thr-52 and Gly-...