Enhancing the stability of Geobacillus zalihae T1 lipase in organic solvents and insights into the structural stability of its variants

• Main Authors: Maiangwa, Jonathan, Hamdan, Siti Hajar, Mohamad Ali, Mohd Shukuri, Salleh, Abu Bakar, Raja Abd Rahman, Raja Noor Zaliha, Mohd Shariff, Fairolniza, Leow, Adam Thean Chor
• Format: Article
• Language: English
• Published: Elsevier 2021
• Online Access: http://psasir.upm.edu.my/id/eprint/97110/1/ABSTRACT.pdf; http://psasir.upm.edu.my/id/eprint/97110/; https://www.sciencedirect.com/science/article/abs/pii/S1093326321000668

Critical to the applications of proteins in non-aqueous enzymatic processes is their structural dynamics in relation to solvent polarity. A pool of mutants derived from Geobacillus zalihae T1 lipase was screened in organic solvents (methanol, ethanol, propanol, butanol and pentanol) resulting in the...