Purification and biochemical characterization of extracellular thermostable lipase from Bacillus sp. strain L2

• Main Authors: Nezhad, Nima Ghahremani, Mohammed Mukred, Abdul Daim, Raja Abd Rahman, Raja Noor Zaliha, Basri, Mahiran, Salleh, Abu Bakar, Leow, Thean Chor
• Format: Article
• Published: Springer Science and Business Media Deutschland GmbH 2024
• Online Access: http://psasir.upm.edu.my/id/eprint/112037/; https://link.springer.com/article/10.1007/s11756-024-01647-z

In the current study, an extracellular thermostable lipase from Bacillus sp. strain L2 was produced and purified through two-steps purifications, including ammonium sulfate precipitation and Heparin-Sepharose affinity chromatography. Then, the optimum pH, optimum temperature, thermostability, the ef...