Cloning, extracellular expression and characterization of a predominant β-CGTase from Bacillus sp. G1 in E. coli
The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully isolated and cloned into Escherichia coli. Analysis of the nucleotide sequence revealed the presence of an open reading frame of 2,109 bp and encoded a 674 amino acid protein. Purified CGTase exhibit...
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Main Authors: | Ong, Rui Min, Goh, Kian Mau, Mahadi, Nor Muhammad, Hassan, Osman, Rahman, Raja Noor Zaliha Raja Abdul, Illias, Rosli Md. |
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Format: | Article |
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Springer Berlin / Heidelberg
2008
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Online Access: | http://eprints.utm.my/id/eprint/7263/ http://dx.doi.org/10.1007/s10295-008-0462-2 |
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