Purification and characterization of recombinant enzyme CGTase G1

• Main Authors: Goh, Kian Mau, Mahadi, Nor Muhammad, Hassan, Osman, Raja Abdul Rahman, Raja Noor Zaliha, Md. Illias, Rosli
• Format: Conference or Workshop Item
• Language: English
• Published: 2005
• Subjects: T Technology (General)
• Online Access: http://eprints.utm.my/id/eprint/5368/1/GohKianMau2005_PurificationAndCharacterizationOfRecombinantEnzyme.pdf; http://eprints.utm.my/id/eprint/5368/

The cyclodextrin glucanotransferase (CGTase) gene from Bacillus sp. G1 was successfully isolated and cloned into Escherichia coli. The CGTase gene was expressed in E.coli and approximately 62% of CGTase enzyme was secreted into the medium. The recombinant CGTase was purified to homogeneity using amm...