Cloning, extracellular expression and characterization of predominant β-CGTase from Bacillus sp. G1 in E. coli

• Main Authors: Ong, Rui Min, Goh, Kian Mau, Mahadi, Nor Muhammad, Hassan, Osman, Raja Abdul Rahman, Raja Noor Zaliha, Md. Illias, Rosli
• Format: Article
• Language: English
• Published: Springer 2008
• Online Access: http://psasir.upm.edu.my/id/eprint/13611/1/Cloning.pdf; http://psasir.upm.edu.my/id/eprint/13611/; http://link.springer.com/article/10.1007/s10295-008-0462-2?view=classic

The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully isolated and cloned into Escherichia coli. Analysis of the nucleotide sequence revealed the presence of an open reading frame of 2,109 bp and encoded a 674 amino acid protein. Purified CGTase exhibit...