Use of megaprimer and overlapping extension pcr (OE-PCR) to mutagenize and enhance cyclodextrin glucosyltransferase (CGTASE) function
Protein engineering is a very useful tool for probing structure–function relationships in proteins. Specifically, site-directed mutagenized proteins can provide useful insights into structural, binding and catalytic mechanisms of a protein, particularly when coupled with crystallization. In this cha...
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| Main Authors: | , , , |
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| Format: | Book Section |
| Published: |
Humana Press Inc.
2017
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/97041/ http://dx.doi.org/10.1007/978-1-4939-6472-7_27 |
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