The effects of reaction conditions on the production of γ-cyclodextrin from tapioca starch by using a novel recombinant engineered CGTase

• Main Authors: Goh, Kian Mau, Mahadi, Nor Muhammad, Hassan, Osman, Abdul Rahman, Raja Noor Zaliha Raja, Illias, Rosli Md
• Format: Article
• Published: Elsevier B.V. 2007
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/7246/; http://dx.doi.org/10.1016/j.molcatb.2007.09.011

A novel mutant enzyme namely H43T CGTase can produce up to 39% γ-cyclodextrin (γ-CD) compared to the native enzyme which produces only 10% γ-CD. The effect of the reaction conditions on γ-CD production was studied using this mutant CGTase. The effects of substrate-buffer combination, starch pret...