Domain replacement to elucidate the role of B domain in cgtase thermostability and activity

The B domain of CGTase has been generally accepted as a domain involved in thermostability. However, limited work has been performed in which entire B domain is substituted with the thermostable counterpart. Using overlap extension PCR, we replaced the B domain of a variant of CGTase Bacillus sp. G1...

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Bibliographic Details
Main Authors: Poh, Hong Goh, Md. Illias, Rosli, Goh, Kian Mau
Format: Article
Published: Elsevier 2012
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Online Access:http://eprints.utm.my/id/eprint/33450/
http://dx.doi.org/10.1016/j.procbio.2012.07.033
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