Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability

• Main Authors: Rohaida, Che Man, Ahmad Fauzi, Ismail, Nazlee Faisal, Ghazali, Siti Fatimah Zaharah, Mohd Fuzi, Rosli, Md. Illias
• Format: Article
• Language: English
• Published: Elsevier 2015
• Subjects: TP Chemical technology
• Online Access: http://umpir.ump.edu.my/id/eprint/9404/1/fkksa-2015-rohaida-Effects%20of%20the%20Immobilization.pdf; http://umpir.ump.edu.my/id/eprint/9404/; http://dx.doi.org/10.1016/j.bej.2015.02.013

The excretion of recombinant enzymes is a preferred approach for protein expression because of the associated high level of expression, low level of proteolysis, ease of purification and more favorable folding environment. However, cell lysis is one of the major drawbacks in the excretion of enzymes...