Domain replacement to elucidate the role of B domain in cgtase thermostability and activity
The B domain of CGTase has been generally accepted as a domain involved in thermostability. However, limited work has been performed in which entire B domain is substituted with the thermostable counterpart. Using overlap extension PCR, we replaced the B domain of a variant of CGTase Bacillus sp. G1...
Saved in:
Main Authors: | Poh, Hong Goh, Md. Illias, Rosli, Goh, Kian Mau |
---|---|
Format: | Article |
Published: |
Elsevier
2012
|
Subjects: | |
Online Access: | http://eprints.utm.my/id/eprint/33450/ http://dx.doi.org/10.1016/j.procbio.2012.07.033 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Rational mutagenesis of cyclodextrin glucanotransferase at the calcium binding regions for enhancement of thermostability
by: Goh, Poh Hong, et al.
Published: (2012) -
From gene to process : CGtase as a role model
by: Md. Illias, , Rosli, et al.
Published: (2008) -
A predominant β-CGTase G1 engineered to elucidate the relationship between protein structure and product specificity
by: Goh, Kin Mau, et al.
Published: (2009) -
Molecular modeling of a predominant β-CGTase G1 and analysis of ionic interaction in CGTase
by: Goh, Kian Mau, et al.
Published: (2008) -
A predominant β-CGTase G1 engineered to elucidate the relationship between protein structure and product specificity
by: Goh, Kian Mau, et al.
Published: (2009)