Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development
The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using...
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Main Authors: | Eugene Boon Beng, Ong,, Theam Soon, Lim,, Amy Amilda, Anthony,, Aziah, Ismail,, Asma, Ismail, |
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Format: | Article |
Language: | English |
Published: |
Elsevier Science Inc
2015
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Subjects: | |
Online Access: | http://ddms.usim.edu.my/handle/123456789/8086 |
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