Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development

• Main Authors: Eugene Boon Beng, Ong,, Theam Soon, Lim,, Amy Amilda, Anthony,, Aziah, Ismail,, Asma, Ismail,
• Format: Article
• Language: English
• Published: Elsevier Science Inc 2015
• Subjects: Salmonella; Typhi; Hlye; Antigen; Immunoblot, ELISA
• Online Access: http://ddms.usim.edu.my/handle/123456789/8086

The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using...