Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development
The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using...
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my.usim-80862015-12-29T08:22:20Z Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development Eugene Boon Beng, Ong, Theam Soon, Lim, Amy Amilda, Anthony, Aziah, Ismail, Asma, Ismail, Salmonella Typhi Hlye Antigen Immunoblot, ELISA The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. (c) 2013 Elsevier Inc. All rights reserved. 2015-05-18T02:34:22Z 2015-05-18T02:34:22Z 2013 Article 0732-8893 http://ddms.usim.edu.my/handle/123456789/8086 en Elsevier Science Inc |
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Salmonella Typhi Hlye Antigen Immunoblot, ELISA |
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Salmonella Typhi Hlye Antigen Immunoblot, ELISA Eugene Boon Beng, Ong, Theam Soon, Lim, Amy Amilda, Anthony, Aziah, Ismail, Asma, Ismail, Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development |
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The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. (c) 2013 Elsevier Inc. All rights reserved. |
format |
Article |
author |
Eugene Boon Beng, Ong, Theam Soon, Lim, Amy Amilda, Anthony, Aziah, Ismail, Asma, Ismail, |
author_facet |
Eugene Boon Beng, Ong, Theam Soon, Lim, Amy Amilda, Anthony, Aziah, Ismail, Asma, Ismail, |
author_sort |
Eugene Boon Beng, Ong, |
title |
Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development |
title_short |
Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development |
title_full |
Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development |
title_fullStr |
Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development |
title_full_unstemmed |
Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development |
title_sort |
cloning, expression, and purification of the hemolysin/cytolysin (hlye antigen) from salmonella enterica serovar typhi: potential application for immunoassay development |
publisher |
Elsevier Science Inc |
publishDate |
2015 |
url |
http://ddms.usim.edu.my/handle/123456789/8086 |
_version_ |
1645152337886445568 |
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13.18916 |