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Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development

The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using...

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Main Authors: Eugene Boon Beng, Ong,, Theam Soon, Lim,, Amy Amilda, Anthony,, Aziah, Ismail,, Asma, Ismail,
Format: Article
Language:English
Published: Elsevier Science Inc 2015
Subjects:
Salmonella
Typhi
Hlye
Antigen
Immunoblot, ELISA
Online Access:http://ddms.usim.edu.my/handle/123456789/8086
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spelling my.usim-80862015-12-29T08:22:20Z Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development Eugene Boon Beng, Ong, Theam Soon, Lim, Amy Amilda, Anthony, Aziah, Ismail, Asma, Ismail, Salmonella Typhi Hlye Antigen Immunoblot, ELISA The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. (c) 2013 Elsevier Inc. All rights reserved. 2015-05-18T02:34:22Z 2015-05-18T02:34:22Z 2013 Article 0732-8893 http://ddms.usim.edu.my/handle/123456789/8086 en Elsevier Science Inc
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Salmonella
Typhi
Hlye
Antigen
Immunoblot, ELISA
spellingShingle Salmonella
Typhi
Hlye
Antigen
Immunoblot, ELISA
Eugene Boon Beng, Ong,
Theam Soon, Lim,
Amy Amilda, Anthony,
Aziah, Ismail,
Asma, Ismail,
Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development
description The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. (c) 2013 Elsevier Inc. All rights reserved.
format Article
author Eugene Boon Beng, Ong,
Theam Soon, Lim,
Amy Amilda, Anthony,
Aziah, Ismail,
Asma, Ismail,
author_facet Eugene Boon Beng, Ong,
Theam Soon, Lim,
Amy Amilda, Anthony,
Aziah, Ismail,
Asma, Ismail,
author_sort Eugene Boon Beng, Ong,
title Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development
title_short Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development
title_full Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development
title_fullStr Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development
title_full_unstemmed Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development
title_sort cloning, expression, and purification of the hemolysin/cytolysin (hlye antigen) from salmonella enterica serovar typhi: potential application for immunoassay development
publisher Elsevier Science Inc
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/8086
_version_ 1645152337886445568
score 13.18916

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