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Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development

The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using...

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Bibliographic Details
Main Authors: Eugene Boon Beng, Ong,, Theam Soon, Lim,, Amy Amilda, Anthony,, Aziah, Ismail,, Asma, Ismail,
Format: Article
Language:English
Published: Elsevier Science Inc 2015
Subjects:
Salmonella
Typhi
Hlye
Antigen
Immunoblot, ELISA
Online Access:http://ddms.usim.edu.my/handle/123456789/8086
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Summary:The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. (c) 2013 Elsevier Inc. All rights reserved.

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