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Purification of the recombinant enhanced green fluorescent protein from Escherichia coli using alcohol + salt aqueous two-phase systems

The enhanced green fluorescent protein (EGFP) is widely used as a marker in life science. Currently, purifications of the EGFP mostly involve chromatographic methods, which are multistep, time-consuming and costly. In the present study, the recombinant EGFP expressed in Escherichia coli was purified...

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Main Authors:	Lo, Sewn Cen, Ramanan, Ramakrishnan Nagasundara, Tey, Beng Ti, Tan, Wen Siang, Show, Pau Loke, Ling, Tau Chuan, Ooi, Chien Wei
格式:	Article
出版:	Elsevier 2018
主题:	Q Science (General) QH Natural history
在线阅读:	http://eprints.um.edu.my/21414/ https://doi.org/10.1016/j.seppur.2017.09.072
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