Purification of the recombinant enhanced green fluorescent protein from Escherichia coli using alcohol + salt aqueous two-phase systems

• Main Authors: Lo, Sewn Cen, Ramanan, Ramakrishnan Nagasundara, Tey, Beng Ti, Tan, Wen Siang, Show, Pau Loke, Ling, Tau Chuan, Ooi, Chien Wei
• Format: Article
• Published: Elsevier 2018
• Subjects: Q Science (General); QH Natural history
• Online Access: http://eprints.um.edu.my/21414/; https://doi.org/10.1016/j.seppur.2017.09.072

The enhanced green fluorescent protein (EGFP) is widely used as a marker in life science. Currently, purifications of the EGFP mostly involve chromatographic methods, which are multistep, time-consuming and costly. In the present study, the recombinant EGFP expressed in Escherichia coli was purified...