Sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant Escherichia coli

• Main Authors: Nelofer, Rubina, Ramanan, Ramakrishnan Nagasundara, Raja Abdul Rahman, Raja Noor Zaliha, Basri, Mahiran, Ariff, Arbakariya
• Format: Article
• Language: English
• Published: Springer 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/22410/1/Sequential%20optimization%20of%20production%20of%20a%20thermostable%20and%20organic%20solvent%20tolerant%20lipase%20by%20recombinant%20Escherichia%20coli.pdf; http://psasir.upm.edu.my/id/eprint/22410/; http://link.springer.com/article/10.1007/s13213-010-0170-9

Several medium formulations were screened for the production of a thermostable and organic solvent tolerant lipase by a recombinant Escherichia coli BL21. The highest lipase production (28.9 ± 4.1 IU/mL) was obtained in Luria Bertani medium with the addition of 1% (w/v) glucose. The medium formulati...