Two-step purification strategy for enhanced recovery of recombinant hepatitis B surface antigen from Pichia pastoris

• Main Authors: Tam, Yew Joon, Allaudin, Zeenathul Nazariah, Rezaei, Morvarid Akhavan, Mohd Lila, Mohd Azmi, Bahaman, Abdul Rani, Lo, Sewn Cen, Tan, Joo Shun, Hani, Homayoun
• Format: Article
• Language: English
• Published: Taylor & Francis 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/15080/1/Two-step%20purification%20strategy%20for%20enhanced%20recovery%20of%20recombinant%20hepatitis%20B%20surface%20antigen%20from%20Pichia%20pastoris.pdf; http://psasir.upm.edu.my/id/eprint/15080/; https://www.tandfonline.com/doi/abs/10.1080/01496395.2015.1135949

Univariate screening on factors affecting the purification performance of recombinant hepatitis B surface antigen (HBsAg) on ion exchange chromatography (IEC) and size exclusion chromatography (SEC) and the establishment of a two-step purification strategy were performed. Amongst four IEC adsorbents...