Purification of the recombinant enhanced green fluorescent protein from Escherichia coli using alcohol + salt aqueous two-phase systems
The enhanced green fluorescent protein (EGFP) is widely used as a marker in life science. Currently, purifications of the EGFP mostly involve chromatographic methods, which are multistep, time-consuming and costly. In the present study, the recombinant EGFP expressed in Escherichia coli was purified...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Published: |
Elsevier
2018
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/21414/ https://doi.org/10.1016/j.seppur.2017.09.072 |
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