Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems

• Main Authors: Lin, Yu Kiat, Ooi, Chien Wei, Tan, Joo Shun, Pau, Loke Show, Ariff, Arbakariya, Ling, Tau Chuan
• Format: Article
• Language: English
• Published: Elsevier 2013
• Online Access: http://psasir.upm.edu.my/id/eprint/28099/1/Recovery%20of%20human%20interferon%20alpha-2b%20from%20recombinant%20Escherichia%20coli%20using%20alcoholsalt-based%20aqueous%20two-phase%20systems.pdf; http://psasir.upm.edu.my/id/eprint/28099/; http://www.sciencedirect.com/science/article/pii/S1383586613005753

The purification of intracellular human recombinant interferon-alpha2b (IFN-α2b) from Escherichia coli (E. coli) was studied using alcohol/salt aqueous two-phase system (ATPS). The influences of nine biphasic systems comprising alcohol-based top phase (ethanol, 1-propanol and 2-propanol) and salt-ba...