Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: types of ion exchange adsorbent and grafted polymer architecture
Hepatitis B virus-like particles expressed in Escherichia coli were purified using anion exchange adsorbents grafted with polymer poly(oligo(ethylene glycol) methacrylate) in flow-through chromatography mode. The virus-like particles were selectively excluded, while the relatively smaller sized host...
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Online Access: | http://psasir.upm.edu.my/id/eprint/73901/1/Size-selective%20purification%20of%20hepatitis%20B%20virus-like%20particle%20in%20flow-through%20chromatography%20types%20of%20ion%20exchange%20adsorbent%20and%20grafted%20polymer%20architecture.pdf http://psasir.upm.edu.my/id/eprint/73901/ https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201700823 |
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my.upm.eprints.739012020-05-17T18:11:34Z http://psasir.upm.edu.my/id/eprint/73901/ Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: types of ion exchange adsorbent and grafted polymer architecture Hon, Wei Ng Fu, Micky Xiang Lee Gek, Kee Chua Gan, Bee Koon Tan, Wen Siang Chien, Wei Ooi Siah, Ying Tang Eng, Seng Chan Beng, Ti Tey Hepatitis B virus-like particles expressed in Escherichia coli were purified using anion exchange adsorbents grafted with polymer poly(oligo(ethylene glycol) methacrylate) in flow-through chromatography mode. The virus-like particles were selectively excluded, while the relatively smaller sized host cell proteins were absorbed. The exclusion of virus-like particles was governed by the accessibility of binding sites (the size of adsorbents and the charge of grafted dextran chains) as well as the architecture (branch-chain length) of the grafted polymer. The branch-chain length of grafted polymer was altered by changing the type of monomers used. The larger adsorbent (90 μm) had an approximately twofold increase in the flow-through recovery, as compared to the smaller adsorbent (30 μm). Generally, polymer-grafted adsorbents improved the exclusion of the virus-like particles. Overall, the middle branch-chain length polymer grafted on larger adsorbent showed optimal performance at 92% flow-through recovery with a purification factor of 1.53. A comparative study between the adsorbent with dextran grafts and the polymer-grafted adsorbent showed that a better exclusion of virus-like particles was achieved with the absorbent grafted with inert polymer. The grafted polymer was also shown to reduce strong interaction between binding sites and virus-like particles, which preserved the particles' structure. Wiley 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73901/1/Size-selective%20purification%20of%20hepatitis%20B%20virus-like%20particle%20in%20flow-through%20chromatography%20types%20of%20ion%20exchange%20adsorbent%20and%20grafted%20polymer%20architecture.pdf Hon, Wei Ng and Fu, Micky Xiang Lee and Gek, Kee Chua and Gan, Bee Koon and Tan, Wen Siang and Chien, Wei Ooi and Siah, Ying Tang and Eng, Seng Chan and Beng, Ti Tey (2018) Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: types of ion exchange adsorbent and grafted polymer architecture. Journal of Separation Science, 41 (10). 2119 - 2129. ISSN 1615-9306; ESSN: 1615-9314 https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201700823 10.1002/jssc.201700823 |
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Hepatitis B virus-like particles expressed in Escherichia coli were purified using anion exchange adsorbents grafted with polymer poly(oligo(ethylene glycol) methacrylate) in flow-through chromatography mode. The virus-like particles were selectively excluded, while the relatively smaller sized host cell proteins were absorbed. The exclusion of virus-like particles was governed by the accessibility of binding sites (the size of adsorbents and the charge of grafted dextran chains) as well as the architecture (branch-chain length) of the grafted polymer. The branch-chain length of grafted polymer was altered by changing the type of monomers used. The larger adsorbent (90 μm) had an approximately twofold increase in the flow-through recovery, as compared to the smaller adsorbent (30 μm). Generally, polymer-grafted adsorbents improved the exclusion of the virus-like particles. Overall, the middle branch-chain length polymer grafted on larger adsorbent showed optimal performance at 92% flow-through recovery with a purification factor of 1.53. A comparative study between the adsorbent with dextran grafts and the polymer-grafted adsorbent showed that a better exclusion of virus-like particles was achieved with the absorbent grafted with inert polymer. The grafted polymer was also shown to reduce strong interaction between binding sites and virus-like particles, which preserved the particles' structure. |
format |
Article |
author |
Hon, Wei Ng Fu, Micky Xiang Lee Gek, Kee Chua Gan, Bee Koon Tan, Wen Siang Chien, Wei Ooi Siah, Ying Tang Eng, Seng Chan Beng, Ti Tey |
spellingShingle |
Hon, Wei Ng Fu, Micky Xiang Lee Gek, Kee Chua Gan, Bee Koon Tan, Wen Siang Chien, Wei Ooi Siah, Ying Tang Eng, Seng Chan Beng, Ti Tey Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: types of ion exchange adsorbent and grafted polymer architecture |
author_facet |
Hon, Wei Ng Fu, Micky Xiang Lee Gek, Kee Chua Gan, Bee Koon Tan, Wen Siang Chien, Wei Ooi Siah, Ying Tang Eng, Seng Chan Beng, Ti Tey |
author_sort |
Hon, Wei Ng |
title |
Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: types of ion exchange adsorbent and grafted polymer architecture |
title_short |
Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: types of ion exchange adsorbent and grafted polymer architecture |
title_full |
Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: types of ion exchange adsorbent and grafted polymer architecture |
title_fullStr |
Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: types of ion exchange adsorbent and grafted polymer architecture |
title_full_unstemmed |
Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: types of ion exchange adsorbent and grafted polymer architecture |
title_sort |
size-selective purification of hepatitis b virus-like particle in flow-through chromatography: types of ion exchange adsorbent and grafted polymer architecture |
publisher |
Wiley |
publishDate |
2018 |
url |
http://psasir.upm.edu.my/id/eprint/73901/1/Size-selective%20purification%20of%20hepatitis%20B%20virus-like%20particle%20in%20flow-through%20chromatography%20types%20of%20ion%20exchange%20adsorbent%20and%20grafted%20polymer%20architecture.pdf http://psasir.upm.edu.my/id/eprint/73901/ https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201700823 |
_version_ |
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13.18916 |