Purification of His-tagged hepatitis B core antigen from unclarified bacterial homogenate using immobilized metal affinity-expanded bed adsorption chromatography

• Main Authors: Yap, Wei Boon, Tey, Beng Ti, Mohammed Alitheen, Noorjahan Banu, Tan, Wen Siang
• Format: Article
• Language: English
• Published: Elsevier 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/11270/1/Purification%20of%20His-tagged%20hepatitis%20B%20core%20antigen%20from%20unclarified%20bacterial%20homogenate%20using%20immobilized%20metal%20affinity-expanded%20bed%20adsorption%20chromatography.pdf; http://psasir.upm.edu.my/id/eprint/11270/

Hepatitis B core antigen (HBcAg) is used as a diagnostic reagent for the detection of hepatitis B virus infection. In this study, immobilized metal affinity-expanded bed adsorption chromatography (IMA-EBAC) was employed to purify N-terminally His-tagged HBcAg from unclarified bacterial homogenate. S...