Antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice
BACKGROUND: Pineapple (Ananas comosus) was demonstrated to be hepatoprotective. This study aims to investigate the reversing effects of pineapple vinegar on paracetamol-induced liver damage in murine model. METHODS: Pineapple juice was fermented via anaerobic and aerobic fermentation to produce...
Saved in:
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BioMed Central
2015
|
Online Access: | http://psasir.upm.edu.my/id/eprint/43800/1/Antioxidant%20effects%20of%20pineapple%20vinegar%20in.pdf http://psasir.upm.edu.my/id/eprint/43800/ |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.upm.eprints.43800 |
---|---|
record_format |
eprints |
spelling |
my.upm.eprints.438002018-04-09T07:47:41Z http://psasir.upm.edu.my/id/eprint/43800/ Antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice Mohamad, Nurul Elyani Swee, Keong Yeap Kian, Lam Lim Mohd Yusof, Hamidah Boon, Kee Beh Sheau, Wei Tan Wan, Yong Ho Sharifuddin, Shaiful Adzni Jamaluddin, Anisah Long, Kamariah Nik Abd Rahman, Nik Mohd Afizan Alitheen, Noorjahan Banu BACKGROUND: Pineapple (Ananas comosus) was demonstrated to be hepatoprotective. This study aims to investigate the reversing effects of pineapple vinegar on paracetamol-induced liver damage in murine model. METHODS: Pineapple juice was fermented via anaerobic and aerobic fermentation to produce pineapple vinegar. Male BALB/c mice (n = 70) were separated into 7 treatment groups (n = 10). Pineapple vinegar (0.08 and 2 mL/kg BW) and synthetic vinegar were used to treat paracetamol-induced liver damage in mice. The hepatoprotective effects were determined by serum biochemistry profiles (aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and triglyceride (TG)), liver antioxidant levels (ferric-reducing ability plasma (FRAP), superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO), and reduced glutathione assays (GSH)) and histopathological examination with hematoxylin and eosin (H&E) staining. The effects were further evaluated by the expression levels of iNOS, NF-κB, and cytochrome P450 2E1 by quantitative real-time PCR and Western blot analyses. Vinegar samples were also tested for in vitro antioxidant (FRAP, 2,2-diphenyl-2-picrylhydrazyl (DPPH), and total phenolic content (TPC)). Soluble phenolic acid contents in the samples were identified by HPLC. RESULTS: Pineapple vinegar contained 169.67 ± 0.05 μg GAE/mL of TPC, with 862.61 ± 4.38 μg/mL gallic acid as the main component. Oral administration of pineapple vinegar at 2 mL/kg BW reduced serum enzyme biomarker levels, including AST (P = 0.008), ALT (P = 0.006), ALP (P = 0.002), and TG (P = 0.006) after 7 days of paracetamol treatment. Liver antioxidant levels such as hepatic glutathione (P = 0.003), SOD (P < 0.001), lipid peroxidation (P = 0.002) and FRAP (P <0.001) were restored after the treatment. Pineapple vinegar reduced the expressions of iNOS (P = 0.003) and NF-kB (P = 0.003) and the level of NO (P = 0.003) significantly. Pineapple vinegar also downregulated liver cytochrome P450 protein expression. CONCLUSIONS: Oral administration of pineapple vinegar at 0.08 and 2 mL/kg BW reduced serum enzyme biomarker levels, restored liver antioxidant levels, reduced inflammatory factor expressions, and down regulated liver cytochrome P450 protein expression in paracetamol-induced liver damage in mice. BioMed Central 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/43800/1/Antioxidant%20effects%20of%20pineapple%20vinegar%20in.pdf Mohamad, Nurul Elyani and Swee, Keong Yeap and Kian, Lam Lim and Mohd Yusof, Hamidah and Boon, Kee Beh and Sheau, Wei Tan and Wan, Yong Ho and Sharifuddin, Shaiful Adzni and Jamaluddin, Anisah and Long, Kamariah and Nik Abd Rahman, Nik Mohd Afizan and Alitheen, Noorjahan Banu (2015) Antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice. Chinese Medicine, 10 (3). pp. 1-10. ISSN 1749-8546 10.1186/s13020-015-0030-4 |
institution |
Universiti Putra Malaysia |
building |
UPM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Putra Malaysia |
content_source |
UPM Institutional Repository |
url_provider |
http://psasir.upm.edu.my/ |
language |
English |
description |
BACKGROUND:
Pineapple (Ananas comosus) was demonstrated to be hepatoprotective. This study aims to investigate the reversing effects of pineapple vinegar on paracetamol-induced liver damage in murine model.
METHODS:
Pineapple juice was fermented via anaerobic and aerobic fermentation to produce pineapple vinegar. Male BALB/c mice (n = 70) were separated into 7 treatment groups (n = 10). Pineapple vinegar (0.08 and 2 mL/kg BW) and synthetic vinegar were used to treat paracetamol-induced liver damage in mice. The hepatoprotective effects were determined by serum biochemistry profiles (aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and triglyceride (TG)), liver antioxidant levels (ferric-reducing ability plasma (FRAP), superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO), and reduced glutathione assays (GSH)) and histopathological examination with hematoxylin and eosin (H&E) staining. The effects were further evaluated by the expression levels of iNOS, NF-κB, and cytochrome P450 2E1 by quantitative real-time PCR and Western blot analyses. Vinegar samples were also tested for in vitro antioxidant (FRAP, 2,2-diphenyl-2-picrylhydrazyl (DPPH), and total phenolic content (TPC)). Soluble phenolic acid contents in the samples were identified by HPLC.
RESULTS:
Pineapple vinegar contained 169.67 ± 0.05 μg GAE/mL of TPC, with 862.61 ± 4.38 μg/mL gallic acid as the main component. Oral administration of pineapple vinegar at 2 mL/kg BW reduced serum enzyme biomarker levels, including AST (P = 0.008), ALT (P = 0.006), ALP (P = 0.002), and TG (P = 0.006) after 7 days of paracetamol treatment. Liver antioxidant levels such as hepatic glutathione (P = 0.003), SOD (P < 0.001), lipid peroxidation (P = 0.002) and FRAP (P <0.001) were restored after the treatment. Pineapple vinegar reduced the expressions of iNOS (P = 0.003) and NF-kB (P = 0.003) and the level of NO (P = 0.003) significantly. Pineapple vinegar also downregulated liver cytochrome P450 protein expression.
CONCLUSIONS:
Oral administration of pineapple vinegar at 0.08 and 2 mL/kg BW reduced serum enzyme biomarker levels, restored liver antioxidant levels, reduced inflammatory factor expressions, and down regulated liver cytochrome P450 protein expression in paracetamol-induced liver damage in mice. |
format |
Article |
author |
Mohamad, Nurul Elyani Swee, Keong Yeap Kian, Lam Lim Mohd Yusof, Hamidah Boon, Kee Beh Sheau, Wei Tan Wan, Yong Ho Sharifuddin, Shaiful Adzni Jamaluddin, Anisah Long, Kamariah Nik Abd Rahman, Nik Mohd Afizan Alitheen, Noorjahan Banu |
spellingShingle |
Mohamad, Nurul Elyani Swee, Keong Yeap Kian, Lam Lim Mohd Yusof, Hamidah Boon, Kee Beh Sheau, Wei Tan Wan, Yong Ho Sharifuddin, Shaiful Adzni Jamaluddin, Anisah Long, Kamariah Nik Abd Rahman, Nik Mohd Afizan Alitheen, Noorjahan Banu Antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice |
author_facet |
Mohamad, Nurul Elyani Swee, Keong Yeap Kian, Lam Lim Mohd Yusof, Hamidah Boon, Kee Beh Sheau, Wei Tan Wan, Yong Ho Sharifuddin, Shaiful Adzni Jamaluddin, Anisah Long, Kamariah Nik Abd Rahman, Nik Mohd Afizan Alitheen, Noorjahan Banu |
author_sort |
Mohamad, Nurul Elyani |
title |
Antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice |
title_short |
Antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice |
title_full |
Antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice |
title_fullStr |
Antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice |
title_full_unstemmed |
Antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice |
title_sort |
antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice |
publisher |
BioMed Central |
publishDate |
2015 |
url |
http://psasir.upm.edu.my/id/eprint/43800/1/Antioxidant%20effects%20of%20pineapple%20vinegar%20in.pdf http://psasir.upm.edu.my/id/eprint/43800/ |
_version_ |
1643833672732770304 |
score |
13.160551 |