A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method
A single-step purification method based on an anion exchange chromatography was developed to purify truncated Nipah virus glycoprotein (tNiVG) expressed in Spodoptera frugiperda 9 (Sf9) insect cells. The preferred buffer conditions to bind and elute tNiVG protein were 50 mM sodium carbonate with pH...
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Main Authors: | Raksha, Sunhare, Tan, Wen Siang, Hamid, Muhajir, Ramanan, Ramakrishnan Nagasundara, Tey, Beng Ti |
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Format: | Article |
Language: | English |
Published: |
Taylor & Francis
2014
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Online Access: | http://psasir.upm.edu.my/id/eprint/36282/1/A%20single.pdf http://psasir.upm.edu.my/id/eprint/36282/ |
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