Purification of rabbit polyclonal immunoglobulin G with ammonium sulphate precipitation and mixed-mode chromatography

• Main Authors: Mariam, S. H. S, Ooi, Chien Wei, Tan, Wen Siang, Abdullah, Janna Ong, Ariff, Arbakariya, Tey, Beng Ti
• Format: Article
• Language: English
• Published: Elsevier 2015
• Online Access: http://psasir.upm.edu.my/id/eprint/46050/1/Purification%20of%20rabbit%20polyclonal%20immunoglobulin%20G%20with%20ammonium%20sulphate%20precipitation%20and%20mixed-mode%20chromatography.pdf; http://psasir.upm.edu.my/id/eprint/46050/; https://www.sciencedirect.com/science/article/pii/S1383586615000969

Immunoglobulins G (IgG) against hepatitis B core antigen (HBcAg) was successfully purified using a purification scheme comprising ammonium sulphate precipitation and SepFast™ MM AH-1 column chromatography. Ammonium sulphate precipitation performed at 40% saturation was optimum in terms of the recove...