Purification of His-tagged hepatitis B core antigen from unclarified bacterial homogenate using immobilized metal affinity-expanded bed adsorption chromatography
Hepatitis B core antigen (HBcAg) is used as a diagnostic reagent for the detection of hepatitis B virus infection. In this study, immobilized metal affinity-expanded bed adsorption chromatography (IMA-EBAC) was employed to purify N-terminally His-tagged HBcAg from unclarified bacterial homogenate. S...
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Main Authors: | Yap, Wei Boon, Tey, Beng Ti, Mohammed Alitheen, Noorjahan Banu, Tan, Wen Siang |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2010
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Online Access: | http://psasir.upm.edu.my/id/eprint/11270/1/Purification%20of%20His-tagged%20hepatitis%20B%20core%20antigen%20from%20unclarified%20bacterial%20homogenate%20using%20immobilized%20metal%20affinity-expanded%20bed%20adsorption%20chromatography.pdf http://psasir.upm.edu.my/id/eprint/11270/ |
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