Optimisation of pre-harvest sago frond sap for the production of llactic acid using Lactococcus lactis IO-1
The major restriction of sago palm plantation is the long maturation period for the starch extraction. As such, alternative commodity has to be identified to increase commercial value of sago palm especially from the by-product. Sago frond has been harvested to have numerous advantages as an altern...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/38941/1/Enzymatic1.pdf http://ir.unimas.my/id/eprint/38941/ https://www.sciencedirect.com/science/article/abs/pii/S1878818122001621 |
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| Summary: | The major restriction of sago palm plantation is the long maturation period for the starch extraction. As such, alternative commodity has to be identified to increase commercial value of sago
palm especially from the by-product. Sago frond has been harvested to have numerous advantages as an alternative substrate for the production of biomaterial and biofuel such as lactic acid
and bioethanol. This study focuses on effective approach to maximise the concentration of sugar
in the substrate and to identify the ideal concentration of sago frond sap (SFSp) as sole media to
produce l-lactic acid and grow L. lactis IO-1. Result shows that SFSp extracted from sago rachis
using a roller press machine contained glucose (43.8 g/L), xylose (17.3 g/L) as free sugars and
starch (5.55 g/L). Combination of enzymatic hydrolysis and sterilisation pre-treatment was found
to maximise the concentration of total carbon source (68 g/L) and sterilised sago frond sap suitable to be used for fermentation process. The ideal concentration of SFSp that was determined at
50% dilution produces the highest amount of l-lactic acid (24.02 g/L) synergist by most efficient
sugar consumption (89.93%). The use of SFSp as fermentation medium for l-lactic acid production generated maximum biomass concentration of 14.53 g/L. The utilisation of SFSp extracted
from pre-harvest sago frond as substrate promote efficiency of l-lactic acid production associated
to exceptional growth performance of L. lactis IO-1. In conclusion, the concept of utilizing byproduct from sago palm as novel raw material promise reliable resources and superiority of L. lactis IO-1 to metabolise sugar in SFSp promote lucrative and ethical production of l-lactic acid. |
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