Quantification of Zonula Occludens-1 mRNA expression in cultured bovine retinal endothelial cells, using SYBR green real-time PCR

• Main Authors: Muniandy, S., Yaiw, K.C., Chook, J.B., Rayappan, A.V., Wong, K.T., Ismail, I.S., Qvist, R.
• Format: Article
• Language: English
• Published: 2008
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/7220/1/Quantification_of_Zonula_Occludens.pdf; http://eprints.um.edu.my/7220/

A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1...