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Quantification of Zonula Occludens-1 mRNA expression in cultured bovine retinal endothelial cells, using SYBR green real-time PCR

A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1...

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Bibliographic Details
Main Authors: Muniandy, S., Yaiw, K.C., Chook, J.B., Rayappan, A.V., Wong, K.T., Ismail, I.S., Qvist, R.
Format: Article
Language:English
Published: 2008
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/7220/1/Quantification_of_Zonula_Occludens.pdf
http://eprints.um.edu.my/7220/
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Summary:A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1 mRNA expression in histamine-treated (10(-7)M to 10(-9) M) and untreated bovine retinal endothelial cells at high magnesium levels. Immunohistochemistry staining and western blot assay unequivocally demonstrated the approximately similar levels of ZO-1 protein in both histamine-treated and non-treated endothelial cells at high magnesium concentrations.

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