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Quantification of Zonula Occludens-1 mRNA expression in cultured bovine retinal endothelial cells, using SYBR green real-time PCR

A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1...

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Main Authors: Muniandy, S., Yaiw, K.C., Chook, J.B., Rayappan, A.V., Wong, K.T., Ismail, I.S., Qvist, R.
Format: Article
Language:English
Published: 2008
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/7220/1/Quantification_of_Zonula_Occludens.pdf
http://eprints.um.edu.my/7220/
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spelling my.um.eprints.72202014-10-28T05:53:48Z http://eprints.um.edu.my/7220/ Quantification of Zonula Occludens-1 mRNA expression in cultured bovine retinal endothelial cells, using SYBR green real-time PCR Muniandy, S. Yaiw, K.C. Chook, J.B. Rayappan, A.V. Wong, K.T. Ismail, I.S. Qvist, R. R Medicine A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1 mRNA expression in histamine-treated (10(-7)M to 10(-9) M) and untreated bovine retinal endothelial cells at high magnesium levels. Immunohistochemistry staining and western blot assay unequivocally demonstrated the approximately similar levels of ZO-1 protein in both histamine-treated and non-treated endothelial cells at high magnesium concentrations. 2008 Article PeerReviewed application/pdf en http://eprints.um.edu.my/7220/1/Quantification_of_Zonula_Occludens.pdf Muniandy, S. and Yaiw, K.C. and Chook, J.B. and Rayappan, A.V. and Wong, K.T. and Ismail, I.S. and Qvist, R. (2008) Quantification of Zonula Occludens-1 mRNA expression in cultured bovine retinal endothelial cells, using SYBR green real-time PCR. Biomedical Research-India, 19 (2). pp. 117-124. ISSN 0970-938X
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Muniandy, S.
Yaiw, K.C.
Chook, J.B.
Rayappan, A.V.
Wong, K.T.
Ismail, I.S.
Qvist, R.
Quantification of Zonula Occludens-1 mRNA expression in cultured bovine retinal endothelial cells, using SYBR green real-time PCR
description A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1 mRNA expression in histamine-treated (10(-7)M to 10(-9) M) and untreated bovine retinal endothelial cells at high magnesium levels. Immunohistochemistry staining and western blot assay unequivocally demonstrated the approximately similar levels of ZO-1 protein in both histamine-treated and non-treated endothelial cells at high magnesium concentrations.
format Article
author Muniandy, S.
Yaiw, K.C.
Chook, J.B.
Rayappan, A.V.
Wong, K.T.
Ismail, I.S.
Qvist, R.
author_facet Muniandy, S.
Yaiw, K.C.
Chook, J.B.
Rayappan, A.V.
Wong, K.T.
Ismail, I.S.
Qvist, R.
author_sort Muniandy, S.
title Quantification of Zonula Occludens-1 mRNA expression in cultured bovine retinal endothelial cells, using SYBR green real-time PCR
title_short Quantification of Zonula Occludens-1 mRNA expression in cultured bovine retinal endothelial cells, using SYBR green real-time PCR
title_full Quantification of Zonula Occludens-1 mRNA expression in cultured bovine retinal endothelial cells, using SYBR green real-time PCR
title_fullStr Quantification of Zonula Occludens-1 mRNA expression in cultured bovine retinal endothelial cells, using SYBR green real-time PCR
title_full_unstemmed Quantification of Zonula Occludens-1 mRNA expression in cultured bovine retinal endothelial cells, using SYBR green real-time PCR
title_sort quantification of zonula occludens-1 mrna expression in cultured bovine retinal endothelial cells, using sybr green real-time pcr
publishDate 2008
url http://eprints.um.edu.my/7220/1/Quantification_of_Zonula_Occludens.pdf
http://eprints.um.edu.my/7220/
_version_ 1643687992964939776
score 13.214268

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