Carotenoid pigments from selected green and blue-green algae species cell culture as potential Halal Food colorants

• Main Authors: Wan Sulaiman, Wan Syibrah Hanisah, Md Zaini, Haslin Hanani, Othman, Rashidi, Mohd Hatta, Farah Ayuni, Ramya, Razanah, Mohd Latiff, Nur Hanie
• Format: Conference or Workshop Item
• Language: English; English
• Published: 2021
• Subjects: RS Pharmacy and materia medica; SH389 Fisheries-Algae and Algae Culture; TP248.13 Biotechnology; TP934 Paints, pigments, varnishes, etc
• Online Access: http://irep.iium.edu.my/95225/1/PAPER%20D185.pdf; http://irep.iium.edu.my/95225/6/ICIABC21%20SLIDE.pdf; http://irep.iium.edu.my/95225/

Halal products are becoming widely recognised as a new standard for quality assurance and safety and also there is a strong need to investigate the potential of natural pigments, particularly carotenoids in microalgae to be fully commercialised especially in the halal market, health advantages, food products and dye technology. Therefore, this research aimed is to explore new sources of pigments as a halal food colourant. A total of 13 species were evaluated for carotenoid profile quantitative and qualitatively, namely, Chlorella fusca, Chlorella vulgaris, Selenastrum capricornutum, Pandorina morum, Botryococcus sudeticus, Botryococcus braunii, Chlorococcum sp., Ankistodesmus sp., Scenedesmus sp., Pseudanabaena sp., Synechococcus sp., Alkalinema sp., and Phormidium sp. The first 9 species are green algae while the remaining 4 are cyanobacteria. The main carotenoids identified through HPLC analysis were β-cryptoxanthin, β-carotene, zeaxanthin, neoxanthin, lutein and violaxanthin which vary in the ratio between species. β-cryptoxanthin was detected substantially higher in Phormidium sp. (30.58 μg/g DW); β-carotene in C. vulgaris (356.15 ug/g DW); zeaxanthin in Synechococcus sp. (7731.30 μg/g DW); neoxanthin in Chlorococcum sp. (129.27 μg/g DW); whereas lutein and violaxanthin in C. fusca (220.14 μg/g DW) and (307.94 μg/g DW) respectively. The research’s significant outcome will be discoveries of new natural carotenoid pigment sources as potential food colorants for the halal industry.