Overexpression, purification and characterization of Aspergillus niger beta-glucosidase in Pichia pastoris

This study describes the expression of β-glucosidase (BglA) from Aspergillus niger in Pichia pastoris, a methylotrophic yeast strain, under the regulation of an alcohol oxidase promoter. The heterologous expression of BglA was optimized in a shake flask. Optimal conditions were achieved using an i...

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Bibliographic Details
Main Authors: Kamaruddin, S., Abu Bakar, F.D., Illias, R.M., Said, M., Hassan, O., Murad, A.M.A.
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2015
Online Access:http://journalarticle.ukm.my/8690/1/44_1_02.pdf
http://journalarticle.ukm.my/8690/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=505&catid=59:current-view&Itemid=56
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