Expression and characterization of a cellobiohydrolase (CBH7B) from the thermophilic fungus Thielavia terrestris in Pichia pastoris

• Main Authors: Woon, J. S. K., Mackeen, M. M., Mahadi, N. M., Illias, R. M., Abdul Murad, A. M., Abu Bakar, F. D.
• Format: Article
• Published: Wiley-Blackwell Publishing Ltd 2016
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/72149/; https://www.scopus.com/inward/record.uri?eid=2-s2.0-84949255331&doi=10.1002%2fbab.1431&partnerID=40&md5=10087f49c35c04b824b5eb690ca5d8c9

The gene encoding a cellobiohydrolase 7B (CBH7B) of the thermophilic fungus Thielavia terrestris was identified, subcloned, and expressed in Pichia pastoris. CBH7B encoded 455 amino acid residues with a molecular mass of 51.8 kDa. Domain analysis indicated that CBH7B contains a family 7 glycosyl hyd...