Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived from Aspergillus flavus NSH9 in Pichia pastoris

• Main Authors: Kazi Muhammad, Rezaul Karim, Ahmad, Husaini, Md. Anowar, Hossain, Ngieng, Ngui Sing, Fazia, Mohd Sinang, Mohd Hasnain, Md Hussain, Hairul Azman, Roslan
• Format: Article
• Language: English
• Published: Hidawi Publishing Coperation 2016
• Subjects: Q Science (General); QD Chemistry
• Online Access: http://ir.unimas.my/id/eprint/12764/1/Kazi%20Muhammad.pdf; http://ir.unimas.my/id/eprint/12764/; https://www.scopus.com/inward/record.uri?eid=2-s2.0-84982796344&partnerID=40&md5=9a99c00cfdab1191cf4cd5bb9858b1e3; http://dx.doi.org/10.1155/2016/5962028

A novel thermostable glucoamylase cDNA without starch binding domain (SBD) of Aspergillus flavus NSH9 was successfully identified, isolated, and overexpressed in Pichia pastoris GS115.The complete open reading frame of glucoamylase from Aspergillus flavus NSH9 was identified by employing PCR that en...