Multiple overlap extension PCR (MOE-PCR): an effective technical shortcut to high throughput synthetic biology

• Main Authors: Kadkhodaei, Saeid, Memari, Hamid Rajabi, Abbasiliasi, Sahar, Rezaei, Morvarid Akhavan, Movahedi, Ali, Tan, Joo Shun, Ariff, Arbakariya
• Format: Article
• Published: Royal Society of Chemistry 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/53351/; http://pubs.rsc.org/en/content/articlelanding/2016/ra/c6ra13172g#!divAbstract

The current study describes multiple-overlap-extension PCR (MOE-PCR) as a simple and effective approach to assembling multiple DNA fragments with various sizes and features in a single in vitro reaction. In this research, 50 bp of homology in overlapping DNA fragments and a specific touchdown PCR pr...