Characterization of the Proteins in Honey
This study focused on the extraction and identification of honey proteins from selected samples (Acacia, Tualang, and Gelam) from Malaysia. The extraction methods, dialysis, ammonium sulfate, and sodium tungstate precipitation methods, were used to obtain the proteins from the honey. The method of m...
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my.utm.580402021-08-22T08:41:21Z http://eprints.utm.my/id/eprint/58040/ Characterization of the Proteins in Honey Lee, Suan Chua Jun, You Lee Giek, Far Chan Q Science (General) This study focused on the extraction and identification of honey proteins from selected samples (Acacia, Tualang, and Gelam) from Malaysia. The extraction methods, dialysis, ammonium sulfate, and sodium tungstate precipitation methods, were used to obtain the proteins from the honey. The method of membrane dialysis provided the highest yield of proteins based on the Bradford assay. This method also provided the highest number of protein bands and the clearest protein bands on a 12% polyacrylamide gel compared with the other two precipitation methods. The results revealed that the precipitation methods may degrade the proteins because of the use of strong chemicals and losses after repeated transfers. Mass spectrometric data showed that the honey contained major royal jelly proteins (MRJP) such as MRJP-1, MRJP-2, MRJP-5, and MRJP-7, as well as a few uncharacterized proteins from Apis mellifera. MRJP-1 was the most abundant protein, particularly in Acacia samples. Honey proteins were also shown to possess significant antioxidant activity as free radical scavengers and reducing agents. Taylor and Francis Inc. 2015 Article PeerReviewed Lee, Suan Chua and Jun, You Lee and Giek, Far Chan (2015) Characterization of the Proteins in Honey. Alytical Letters, 48 (4). pp. 697-709. ISSN 0003-2719 http://www.tandfonline.com/doi/abs/10.1080/00032719.2014.952374 DOI:10.1080/00032719.2014.952374 |
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Q Science (General) Lee, Suan Chua Jun, You Lee Giek, Far Chan Characterization of the Proteins in Honey |
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This study focused on the extraction and identification of honey proteins from selected samples (Acacia, Tualang, and Gelam) from Malaysia. The extraction methods, dialysis, ammonium sulfate, and sodium tungstate precipitation methods, were used to obtain the proteins from the honey. The method of membrane dialysis provided the highest yield of proteins based on the Bradford assay. This method also provided the highest number of protein bands and the clearest protein bands on a 12% polyacrylamide gel compared with the other two precipitation methods. The results revealed that the precipitation methods may degrade the proteins because of the use of strong chemicals and losses after repeated transfers. Mass spectrometric data showed that the honey contained major royal jelly proteins (MRJP) such as MRJP-1, MRJP-2, MRJP-5, and MRJP-7, as well as a few uncharacterized proteins from Apis mellifera. MRJP-1 was the most abundant protein, particularly in Acacia samples. Honey proteins were also shown to possess significant antioxidant activity as free radical scavengers and reducing agents. |
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Article |
author |
Lee, Suan Chua Jun, You Lee Giek, Far Chan |
author_facet |
Lee, Suan Chua Jun, You Lee Giek, Far Chan |
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Lee, Suan Chua |
title |
Characterization of the Proteins in Honey |
title_short |
Characterization of the Proteins in Honey |
title_full |
Characterization of the Proteins in Honey |
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Characterization of the Proteins in Honey |
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Characterization of the Proteins in Honey |
title_sort |
characterization of the proteins in honey |
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Taylor and Francis Inc. |
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2015 |
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http://eprints.utm.my/id/eprint/58040/ http://www.tandfonline.com/doi/abs/10.1080/00032719.2014.952374 |
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