Potential use of Fourier transform infrared spectroscopy for differentiation of bovine and porcine gelatins

• Main Authors: D. M., Hashim,, Y. B. Che, Man,, R., Norakasha,, M., Shuhaimi,, Z. A., Syahariza,, Y., Salmah,
• Format: Article
• Language: English
• Published: Elsevier Sci Ltd 2015
• Subjects: Attenuated total reflectance (ATR); Discriminant analysis; Fourier transform infrared (FTIR) spectroscopy; Gelatin; Cooman's plot
• Online Access: http://ddms.usim.edu.my/handle/123456789/8370; http://ac.els-cdn.com/S0308814609007043/1-s2.0-S0308814609007043-main.pdf?_tid=b83c8a7a-aea7-11e5-afdb-00000aab0f02&acdnat=1451447287_24eaa4a189b608774a9b1eb665bf1562

In order to classify unknown gelatin into their species of origin, a simple and rapid method for the qualitative determination was developed using Fourier transform infrared (FTIR) in combination with attenuated total reflectance (ATR) and discriminant analysis. The spectra were analysed using a chemometric method, principal component analysis (PCA), to classify and characterise gelatin compounds using regions of the FTIR spectra in the range of 3290-3280 cm(-1) and 1660-1200 cm(-1) as calibration models. Results from PCA, which were subsequently represented by the Cooman's plot showed a clear distinction between gelatin samples of bovine and porcine origins. This qualitative approach, besides providing a rapid determination of the source of gelatin, may also be established based on a second derivative study of the FTIR spectrum to alleviate any doubt of the gelatin source for applications in the food and pharmaceutical industries. (C) 2009 Elsevier Ltd. All rights reserved.