Potential use of Fourier transform infrared spectroscopy for differentiation of bovine and porcine gelatins
In order to classify unknown gelatin into their species of origin, a simple and rapid method for the qualitative determination was developed using Fourier transform infrared (FTIR) in combination with attenuated total reflectance (ATR) and discriminant analysis. The spectra were analysed using a che...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier Sci Ltd
2015
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| Subjects: | |
| Online Access: | http://ddms.usim.edu.my/handle/123456789/8370 http://ac.els-cdn.com/S0308814609007043/1-s2.0-S0308814609007043-main.pdf?_tid=b83c8a7a-aea7-11e5-afdb-00000aab0f02&acdnat=1451447287_24eaa4a189b608774a9b1eb665bf1562 |
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| Summary: | In order to classify unknown gelatin into their species of origin, a simple and rapid method for the qualitative determination was developed using Fourier transform infrared (FTIR) in combination with attenuated total reflectance (ATR) and discriminant analysis. The spectra were analysed using a chemometric method, principal component analysis (PCA), to classify and characterise gelatin compounds using regions of the FTIR spectra in the range of 3290-3280 cm(-1) and 1660-1200 cm(-1) as calibration models. Results from PCA, which were subsequently represented by the Cooman's plot showed a clear distinction between gelatin samples of bovine and porcine origins. This qualitative approach, besides providing a rapid determination of the source of gelatin, may also be established based on a second derivative study of the FTIR spectrum to alleviate any doubt of the gelatin source for applications in the food and pharmaceutical industries. (C) 2009 Elsevier Ltd. All rights reserved. |
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