Changes of thermostability, organic solvent, and pH stability in Geobacillus zalihae HT1 and its mutant by calcium ion

• Main Authors: Ishak, Siti Nor Hasmah, Masomian, Malihe, Ahmad Kamarudin, Nor Hafizah, Mohamad Ali, Mohd Shukuri, Leow, Adam Thean Chor, Raja Abdul Rahman, Raja Noor Zaliha
• Format: Article
• Language: English
• Published: MDPI 2019
• Online Access: http://psasir.upm.edu.my/id/eprint/38365/1/38365.pdf; http://psasir.upm.edu.my/id/eprint/38365/; https://www.mdpi.com/1422-0067/20/10/2561

Thermostable T1 lipase from Geobacillus zalihae has been crystallized using counter-diffusion method under space and Earth conditions. The comparison of the three-dimensional structures from both crystallized proteins show differences in the formation of hydrogen bond and ion interactions. Hydrogen...