mRNA expression of Somatostatin receptors 1-5 in MCF7 and MDA-MB231 breast cancer cells

• Main Authors: Mahmoud, Alkhansa, Mancuso, María Teresa, Tanno, Barbara, Abu Bakar, Md Zuki, Hamzah, Hazilawati, Mohd Noor, Mohd Hezmee
• Format: Article
• Published: Radiance Bahu - Uddeshiya Sanstha 2021
• Online Access: http://psasir.upm.edu.my/id/eprint/94283/; https://ijcrr.com/abstract.php?article_id=3497

Background: Breast cancer is one of the most common types of cancer and heterogeneous disease. Somatostatin receptors (SSTRs) are expressed in normal and tumour tissues including breast cancer cells. Objective: The aim of this study to evaluate the expression of SSTRs subtypes 1 – 5 in MCF7 and MDA-MB231 human breast cancer cell lines and compare the levels between two cells. Methods: The detection of mRNA expression levels of SSTRs in MCF7 and MDA-MB231 was performed using quantitativepolymerase chain reaction (qPCR). Results: SSTR1, 2, 3 and 4 mRNA levels were significantly higher in MDA-MB-231 about MCF-7. The expression of SSTR 4 mRNA was highest in MDA-MB231 and MCF-7 cell lines, however, SSTR3 mRNA was least expressed in both cell lines. Conclusion: SSTRs subtypes (1 – 5) were expressed in both MDA-MB231 and MCF7 cells. However, the levels of expression differ between both cell lines.