Antioxidant and anti-obesity properties of local chilies varieties in Malaysia
Obesity is a serious health concern as it may initiate common chronic diseases. Chili pepper is an important spice that brings spiciness and commonly used in cuisines. However, the antioxidant and anti-obesity properties of chili varieties in Malaysia has not yet been fully investigated. Therefore,...
Saved in:
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Springer Science
2020
|
Online Access: | http://psasir.upm.edu.my/id/eprint/87709/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/87709/ https://link.springer.com/article/10.1007/s13197-020-04400-x |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.upm.eprints.87709 |
---|---|
record_format |
eprints |
spelling |
my.upm.eprints.877092022-07-06T02:56:23Z http://psasir.upm.edu.my/id/eprint/87709/ Antioxidant and anti-obesity properties of local chilies varieties in Malaysia Chan, Suk Huei Azlan, Azrina Ismail, Amin Shafie, Nurul Husna Sultana, Sharmin Obesity is a serious health concern as it may initiate common chronic diseases. Chili pepper is an important spice that brings spiciness and commonly used in cuisines. However, the antioxidant and anti-obesity properties of chili varieties in Malaysia has not yet been fully investigated. Therefore, this study aimed to determine the antioxidant (content and activity) and anti-obesity properties of five different varieties of local chili peppers. The antioxidant activities of the extracts were determined through ferric-reducing antioxidant power (FRAP) and 2, 2'azinobis-(3-ethyl-benzothiazoline-6-sulphonic acid (ABTS) assays. Cell cytotoxicity of the selected chili extracts was determined in 3T3-L1 pre adipocytes using cell viability assay (MTT) assay. Whereas the ability to inhibit oil accumulation in fully differentiated 3T3-L1 adipocytes of the selected chili pepper extracts was assayed using Oil Red O staining. The results showed that Kulai 568 pulp extract had the highest level of total phenolic content (TPC) (47.88 ± 0.220 mg GAE/g), whereas Centil pulp extract had the highest level of total flavonoid content (TFC) (26.60 ± 0.52 mg QE/g). In term of antioxidant activities, Bara pulp extract had the highest value in FRAP (3.058 ± 0.002 mM Fe2+/mg extract) and ABTS (IC50 = 12.411 ± 0.025). High performance liquid chromatography (HPLC) analysis, Bara pulp extract has the highest level of capsaicin (72.271 ± 0.957 µg/ml). In terms of inhibition of oil accumulation Centil seed extract presented the best result (69.09-92.20%), while Bara pulp extract inhibited the most pancreatic lipase activity (IC50 = 4.84 ± 0.57 µg/ml). Thus, it is suggested that Centil seed and Bara pulp extracts can be a potent antioxidant and anti-obesity agents. Springer Science 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87709/1/ABSTRACT.pdf Chan, Suk Huei and Azlan, Azrina and Ismail, Amin and Shafie, Nurul Husna and Sultana, Sharmin (2020) Antioxidant and anti-obesity properties of local chilies varieties in Malaysia. Journal of Food Science and Technology, 57 (10). 3677 - 3687. ISSN 0022-1155; ESSN: 0975-8402 https://link.springer.com/article/10.1007/s13197-020-04400-x 10.1007/s13197-020-04400-x |
institution |
Universiti Putra Malaysia |
building |
UPM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Putra Malaysia |
content_source |
UPM Institutional Repository |
url_provider |
http://psasir.upm.edu.my/ |
language |
English |
description |
Obesity is a serious health concern as it may initiate common chronic diseases. Chili pepper is an important spice that brings spiciness and commonly used in cuisines. However, the antioxidant and anti-obesity properties of chili varieties in Malaysia has not yet been fully investigated. Therefore, this study aimed to determine the antioxidant (content and activity) and anti-obesity properties of five different varieties of local chili peppers. The antioxidant activities of the extracts were determined through ferric-reducing antioxidant power (FRAP) and 2, 2'azinobis-(3-ethyl-benzothiazoline-6-sulphonic acid (ABTS) assays. Cell cytotoxicity of the selected chili extracts was determined in 3T3-L1 pre adipocytes using cell viability assay (MTT) assay. Whereas the ability to inhibit oil accumulation in fully differentiated 3T3-L1 adipocytes of the selected chili pepper extracts was assayed using Oil Red O staining. The results showed that Kulai 568 pulp extract had the highest level of total phenolic content (TPC) (47.88 ± 0.220 mg GAE/g), whereas Centil pulp extract had the highest level of total flavonoid content (TFC) (26.60 ± 0.52 mg QE/g). In term of antioxidant activities, Bara pulp extract had the highest value in FRAP (3.058 ± 0.002 mM Fe2+/mg extract) and ABTS (IC50 = 12.411 ± 0.025). High performance liquid chromatography (HPLC) analysis, Bara pulp extract has the highest level of capsaicin (72.271 ± 0.957 µg/ml). In terms of inhibition of oil accumulation Centil seed extract presented the best result (69.09-92.20%), while Bara pulp extract inhibited the most pancreatic lipase activity (IC50 = 4.84 ± 0.57 µg/ml). Thus, it is suggested that Centil seed and Bara pulp extracts can be a potent antioxidant and anti-obesity agents. |
format |
Article |
author |
Chan, Suk Huei Azlan, Azrina Ismail, Amin Shafie, Nurul Husna Sultana, Sharmin |
spellingShingle |
Chan, Suk Huei Azlan, Azrina Ismail, Amin Shafie, Nurul Husna Sultana, Sharmin Antioxidant and anti-obesity properties of local chilies varieties in Malaysia |
author_facet |
Chan, Suk Huei Azlan, Azrina Ismail, Amin Shafie, Nurul Husna Sultana, Sharmin |
author_sort |
Chan, Suk Huei |
title |
Antioxidant and anti-obesity properties of local chilies varieties in Malaysia |
title_short |
Antioxidant and anti-obesity properties of local chilies varieties in Malaysia |
title_full |
Antioxidant and anti-obesity properties of local chilies varieties in Malaysia |
title_fullStr |
Antioxidant and anti-obesity properties of local chilies varieties in Malaysia |
title_full_unstemmed |
Antioxidant and anti-obesity properties of local chilies varieties in Malaysia |
title_sort |
antioxidant and anti-obesity properties of local chilies varieties in malaysia |
publisher |
Springer Science |
publishDate |
2020 |
url |
http://psasir.upm.edu.my/id/eprint/87709/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/87709/ https://link.springer.com/article/10.1007/s13197-020-04400-x |
_version_ |
1738511969523073024 |
score |
13.160551 |