Determination of cell viability using acridine orange/propidium iodide dual-spectrofluorometry assay

• Main Authors: Hussain, Husniza, Santhana, Raj L., Ahmad, Syahida, Abd Razak, Mohd Fuat, Wan Mohamud, Wan Nazaimoon, Bakar, Jamilah, Mohd. Ghazali, Hasanah, Yildiz, Fatih
• Format: Article
• Language: English
• Published: Taylor & Francis 2019
• Online Access: http://psasir.upm.edu.my/id/eprint/81120/1/CELL.pdf; http://psasir.upm.edu.my/id/eprint/81120/; https://www.tandfonline.com/loi/oafa20

In vitro cell viability tests are usually done using 3-(4,5 dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) method. A new spectrofluorometry method was developed using acridine orange (AO) and propidium iodide (PI) using multi-label microplate reader. Nine bio...