Cold-adapted RTX lipase from antarctic Pseudomonas sp. strain AMS8: isolation, molecular modeling and heterologous expression

• Main Authors: Mohamad Ali, Mohd Shukuri, Ganasen, Menega, Raja Abdul Rahman, Raja Noor Zaliha, Leow, Adam Thean Chor, Salleh, Abu Bakar, Basri, Mahiran
• Format: Article
• Language: English
• Published: Springer 2013
• Online Access: http://psasir.upm.edu.my/id/eprint/28129/1/Cold.pdf; http://psasir.upm.edu.my/id/eprint/28129/; http://link.springer.com/article/10.1007/s10930-013-9488-z?view=classic

A new strain of psychrophilic bacteria (designated strain AMS8) from Antarctic soil was screened for extracellular lipolytic activity and further analyzed using molecular approach. Analysis of 16S rDNA showed that strain AMS8 was similar to Pseudomonas sp. A lipase gene named lipAMS8 was successfull...